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	Provedor de dados BABT (1) BJM (1) BJPS (1) Autor Charousová,Ivana (3) Medo,Juraj (3) Hleba,Lukáš (2) Javoreková,Soňa (2) Císarová,Miroslava (1) Javoreková,Sona (1) Steinmetz,Heinrich (1) Wink,Joachim (1) Mais... Palavra-chave 16S rRNA (2) MALDI-TOF MS (2) Streptomyces (2) Actinomycin/antimicrobial activity (1) Anticandidal activity (1) Antimycins antibiotics (1) Factumycin (1) Metabolite production (1) Soil (1) Streptomycetes (1) Tetrangomycin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2019 (1) 2018 (1) 2016 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa BJPS Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Císarová,Miroslava; Javoreková,Soňa. In the present study we reported the antimicrobial activity of actinomycetes isolated from aridic soil sample collected in Karoo, South Africa. Eighty-six actinomycete strains were isolated and purified, out of them thirty-four morphologically different strains were tested for antimicrobial activity. Among 35 isolates, 10 (28.57%) showed both antibacterial and antifungal activity. The ethyl acetate extract of strain KRG-1 showed the strongest antimicrobial activity and therefore was selected for further investigation. The almost complete nucleotide sequence of the 16S rRNA gene as well as distinctive matrix-assisted laser desorption/ionization-time-of-flight/mass spectrometry (MALDI-TOF/MS) profile of whole-cell proteins acquired for strain KRG-1 led to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycin/antimicrobial activity; Streptomyces; Soil; 16S rRNA; MALDI-TOF MS. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100594 Characterization of Antimycins – Producing Streptomycete Strain VY46 Isolated from Slovak Soil BABT Charousová,Ivana; Steinmetz,Heinrich; Medo,Juraj; Javoreková,Sona; Wink,Joachim. ABSTRACT The strain no. VY46 was isolated from agricultural soil of Slovak republic and tested for potential antimicrobial activity against various human pathogens. On the basis of results, strain VY46 significantly inhibited growth of yeast Candida albicans and therefore was used for further characterization. In order to explore the potential bioactivities, extract of the fermented broth culture was prepared with organic solvent extraction method. The ethylacetate extract was subjected to HPLC fractionation against Candida albicans and followed by LC/MS analysis for potential production of anticandidal substances. The analysis resulted in the identification of two antimycins antibiotics, which may be responsible for important anticandidal activity of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomycetes; Anticandidal activity; Metabolite production; Antimycins antibiotics. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100349 Streptomyces globosus DK15 and Streptomyces ederensis ST13 as new producers of factumycin and tetrangomycin antibiotics BJM Charousová,Ivana; Medo,Juraj; Hleba,Lukáš; Javoreková,Soňa. ABSTRACT Fifty seven soil-borne actinomycete strains were assessed for the antibiotic production. Two of the most active isolates, designed as Streptomyces ST-13 and DK-15 exhibited a broad range of antimicrobial activity and therefore they were selected for HPLC fractionation against the most suppressed bacteria Staphylococcus aureus (ST-13) and Chromobacterium violaceum (DK-15). LC/MS analysis of extracts showed the presence of polyketides factumycin (DK15) and tetrangomycin (ST13). The taxonomic position of the antibiotic-producing actinomycetes was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolates matched those described for members of the genus Streptomyces. DK-15 strain exhibited... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces; 16S rRNA; MALDI-TOF MS; Tetrangomycin; Factumycin. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400816 Registros recuperados: 3 Primeira ... 1 ... Última

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